Plant Transcription Factor Database
Previous version: v3.0
Brassica oleracea
SBP Family
Species TF ID Description
XP_013584300.1SBP family protein
XP_013585827.1SBP family protein
XP_013585828.1SBP family protein
XP_013586419.1SBP family protein
XP_013586420.1SBP family protein
XP_013586421.1SBP family protein
XP_013586422.1SBP family protein
XP_013586423.1SBP family protein
XP_013586424.1SBP family protein
XP_013586425.1SBP family protein
XP_013586426.1SBP family protein
XP_013588609.1SBP family protein
XP_013588610.1SBP family protein
XP_013590493.1SBP family protein
XP_013590494.1SBP family protein
XP_013590498.1SBP family protein
XP_013591771.1SBP family protein
XP_013591772.1SBP family protein
XP_013591803.1SBP family protein
XP_013593075.1SBP family protein
XP_013593076.1SBP family protein
XP_013593077.1SBP family protein
XP_013593078.1SBP family protein
XP_013593079.1SBP family protein
XP_013596023.1SBP family protein
XP_013598135.1SBP family protein
XP_013598136.1SBP family protein
XP_013598137.1SBP family protein
XP_013598138.1SBP family protein
XP_013598139.1SBP family protein
XP_013598140.1SBP family protein
XP_013598141.1SBP family protein
XP_013598142.1SBP family protein
XP_013598143.1SBP family protein
XP_013598145.1SBP family protein
XP_013598146.1SBP family protein
XP_013601038.1SBP family protein
XP_013607390.1SBP family protein
XP_013607391.1SBP family protein
XP_013607393.1SBP family protein
XP_013610197.1SBP family protein
XP_013610600.1SBP family protein
XP_013612712.1SBP family protein
XP_013612713.1SBP family protein
XP_013612714.1SBP family protein
XP_013613694.1SBP family protein
XP_013618176.1SBP family protein
XP_013618177.1SBP family protein
XP_013618178.1SBP family protein
XP_013620809.1SBP family protein
XP_013623320.1SBP family protein
XP_013623321.1SBP family protein
XP_013625741.1SBP family protein
XP_013626500.1SBP family protein
XP_013626636.1SBP family protein
XP_013632192.1SBP family protein
XP_013635977.1SBP family protein
XP_013635978.1SBP family protein
XP_013635979.1SBP family protein
XP_013635980.1SBP family protein
XP_013636721.1SBP family protein
XP_013637069.1SBP family protein
XP_013637096.1SBP family protein
XP_013637567.1SBP family protein
XP_013638829.1SBP family protein
SBP Family Introduction

SQUAMOSA promoter binding proteins (SBPs) form a major family of plant-specific transcription factors related to flower development. Although SBPs are heterogeneous in primary structure, they share a highly conserved DNA-binding domain (DBD) that has been suggested to be zinc binding. Here we report the NMR solution structures of DBDs of two SBPs of Arabidopsis thaliana, SPL4 and SPL7. The two share essentially the same structural features. Each structure contains two zinc-binding sites consisting of eight Cys or His residues in a Cys3HisCys2HisCys or Cys6HisCys sequence motif in which the first four residues coordinate to one zinc and the last four coordinate to the other. These structures are dissimilar to other known zinc-binding structures, and thus represent a novel type of zinc-binding motif. The electrostatic profile on the surface suggested that a continuous region, including all the conserved basic residues, is involved in the DNA binding, the mode of which is likely to be novel as well.

Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Nunokawa E, Ishizuka Y, Terada T, Shirouzu M, Osanai T, Tanaka A, Seki M, Shinozaki K, Yokoyama S.
A novel zinc-binding motif revealed by solution structures of DNA-binding domains of Arabidopsis SBP-family transcription factors
J Mol Biol. 2004 Mar 12;337(1):49-63.
PMID: 15001351

SBP-box genes were first characterized as SQUAMOSA promoter binding proteins (SBPs) to regulate the expression of MADS-box genes in early flower development of A. majus (Klein et al., 1996). Since then, SBP-box genes have been identified in many plants including green alga, moss, silver birch, A. majus, Arabidopsis and maize. They play critical roles in regulating flower and fruit development as well as other physiological processes ([Moreno et al., 1997],[Eriksson et al., 2004], [Lannenpaa et al., 2004], [Arazi et al., 2005] and [Kropat et al., 2005]). It has been reported that Arabidopsis SPL3, SPL8 and SPL14 involves in flowering, sporogenesis, GA signaling and toxin resistance ([Cardon et al., 1997], [Unte et al., 2003], [Stone et al., 2005] and [Zhang et al., 2006]) while maize tga1 and tomato LeSPL-CNR affect fruit development ([Wang et al., 2005a] and [Manning et al., 2006]).

SBP-box genes encode proteins sharing a conserved DNA-binding domain of 79 amino acid residues. It has been proved that the DNA-binding domain of SBP-box genes is necessary and sufficient to bind to a palindromic GTAC core motif ([Klein et al., 1996], [Cardon et al., 1997], [Cardon et al., 1999], [Lannenpaa et al., 2004] and [Birkenbihl et al., 2005]). Studies on the NMR solution structure of the fragment of Arabidopsis SPL4 and SPL7 revealed that the DNA-binding domain of SBPs consisted of two separate zinc-binding sites. One zinc finger is C3H or C4 and the other is C2HC (Yamasaki et al., 2004 K. Yamasaki et al.,).

Guo AY, Zhu QH, Gu X, Ge S, Yang J, Luo J.
Genome-wide identification and evolutionary analysis of the plant specific SBP-box transcription factor family.
Gene. 2008 Jul 15;418(1-2):1-8.
PMID: 18495384